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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
IFN-alpha ELISA
IFN-alpha ELISA from R&D Systems, a Bio-Techne Brand
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Citations:
(36)
Supplier Page
Supplier Page from
R&D Systems, a Bio-Techne Brand for
IFN-alpha ELISA
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Product Specs
Company Info
Citations
Reviews
Bench Tips
Product Specs
Item
IFN-alpha ELISA
Company
R&D Systems, a Bio-Techne Brand
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
42120-1
Detection Range
12.50 - 400 pg/mL
Reactivity
Mouse
Sensitivity
12.5 pg/mL
Detection Target
IFN-alpha
Quantity
1 Kit (also 1 Pack (5 Plates))
Applications
ELISA
Brand/Trademark
R&D Systems, a Bio-Techne Brand
Assay Time
27.25 hours
NCBI Full Gene Name
interferon alpha 2
NCBI Gene Aliases
IFN-alpha-2, IFN-alphaA, IFNA, IFNA2B, leIF A
Sample Type
Cell Culture Supernates (100 uL)
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date
Specifications/Features
Available conjugates:
Biotin
Specificity:
Mouse IFN-alpha
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R&D Systems, a Bio-Techne Brand
614 McKinley Place N.E.
Minneapolis
,
Minnesota
55413
United States
Phone
: 1 (800) 343-7475
1 (612) 379-2956
Fax: 1 (612) 656-4400
Company Profile
Website:
http://www.rndsystems.com
Citations (36)
(1)
Targeting immunosuppressive Ly6C+ classical monocytes reverses anti-PD-1/CTLA-4 immunotherapy resistance
Frontiers in Immunology
June 28, 2023
B. Leticia Rodriguez, Limo Chen, Yanli Li, Shucheng Miao, David H. Peng, Jared J. Fradette, Lixia Diao, Jessica M. Konen, Frank R. Rojas Alvarez, Luisa M. Solis,
et al.
or
co-culture
supernatants were used to perform
ELISA
assays
with the kits for IFN-α (
R&D Systems
,
42120-1
), IFN-γ (
eBioscience
,
88-7314-88
), TNF-α (
eBioscience
,
88-7324-86
),
IL-2
(
Thermo Fisher Scientific
,
(2)
Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling
Cell proliferation
December 1, 2022
Tu Q, Li Y, Zhu J, Guo L, Liu C, Liu L, Yuan Y, Zou Y, Chen F, Yao L,
et al
.
Quantikine ELISA Kit (
M1000B
), Mouse IFN‐beta Quantikine ELISA Kit (
MIFNB0
) and Mouse IFN‐alpha ELISA Kit (
42120‐1
), Human IL‐10 Quantikine ELISA Kit (
D1000B
), Human IL‐12 p70 Quantikine ELISA Kit (
D1200
) were purchased
(3)
Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine
Nature immunology
April 1, 2022
Li C, Lee A, Grigoryan L, Arunachalam PS, Scott MKD, Trisal M, Wimmers F, Sanyal M, Weidenbacher PA, Feng Y,
et al
.
with the Quantikine ELISA kit (
R&D Systems
,
MIF00
). IFNα in serum was measured using
ELISA
sets (
PBL
,
421201
).
HMGB1
was detected with the Chemiluminescence ELISA kit (
Novus Biologicals
,
NBP262782
). ELISAs were
(4)
EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion
Cell
January 6, 2022
Marzio A, Kurz E, Sahni JM, Di Feo G, Puccini J, Jiang S, Hirsch CA, Arbini AA, Wu WL, Pass HI,
et al
.
Commercial
Assays
Alamar blue,
Biorad
,cat. No.
BUF012B
Mouse IFN-alpha ELISA Kit,
R&D Systems
,cat. No.
42120-1
Deposited Data,Deposited Data,Deposited Data Original images of
western blot
data,This study; Mendeley
(5)
IL-33/ST2 Activation Is involved in Ro60-Regulated Photosensitivity in Cutaneous Lupus Erythematosus
Mediators of inflammation
January 1, 2022
Song Y, Wei F, Liu Y, Han F, Ma L, Zhuang Y, Pan C, Jia Z, Gong A.
addition, IFN-α level in the J774.2 supernatant was determined by using the Mouse IFN-alpha ELISA Kit
42120-1
(
R&D Systems
). 2.12. Statistical Processing GraphPad Prism performed data statistical analysis. All
(6)
Targeting DDX3X triggers anti-tumor immunity via a dsRNA-mediated tumor-intrinsic type I interferon response
Cancer Research
July 1, 2021
Hyeongjwa Choi et al.
PBL assay science
), Human IFN-β (
DIFNB0
,
R&D systems
), Human IFN-γ (
DY285B
,
R&D systems
),
Mouse
IFN
-α (
42120-1
,
PBL assay science
),
Mouse
IFN-β (
DY8234–05
,
R&D systems
), and
Mouse
IFN-γ (
DY485–05
,
R&D systems
) ELISA
(7)
Tumor cell intrinsic RON signaling suppresses innate immune responses in breast cancer through inhibition of IRAK4 signaling.
Cancer Letters
April 1, 2021
Jennifer R. Bourn, Sasha J. Ruiz-Torres, Brian G. Hunt, Nancy M. Benight, Susan E. Waltz
for analysis following the manufacturers protocol for the murine IFNα and IFNβ VeriKine ELISA kits (#
42120-1
and #
42400-1
;
R&D Systems, Inc
., Minneapolis, MN, USA). IFNα and IFNβ concentration (pg/ml) was measured
(8)
MicroRNA-129-5p alleviates spinal cord injury in mice via suppressing the apoptosis and inflammatory response through HMGB1/TLR4/NF-κB pathway
Bioscience reports
March 27, 2020
Wan G, An Y, Tao J, Wang Y, Zhou Q, Yang R, Liang Q.
Inc, Munich, Germany) was used to quantify fluorescent
staining
.
ELISA
The levels of IFN-α (cat. no.
42120-1
),
Mouse
IL-1β (cat. no.
42400-1
), IL-6 (cat. no.
D6050
) and IL-10 (cat. no.
DY417
) in
mice
serum and
(9)
Ultra-thermostable RNA nanoparticles for solubilizing and high-yield loading of paclitaxel for breast cancer therapy
Nature communications
February 20, 2020
Guo S, Vieweger M, Zhang K, Yin H, Wang H, Li X, Li S, Hu S, Sparreboom A, Evers BM,
et al
.
(10)
Rupintrivir reduces RV-induced T
H
-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo
Respiratory research
October 22, 2019
Danov O, Lasswitz L, Obernolte H, Hesse C, Braun A, Wronski S, Sewald K.
(11)
The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses
Cell reports
May 14, 2019
Wang K, Ma H, Liu H, Ye W, Li Z, Cheng L, Zhang L, Lei Y, Shen L, Zhang F.
(12)
Pulmonary phagocyte-derived NPY controls the pathology of severe influenza virus infection
Nature microbiology
February 1, 2019
Fujiwara S, Hoshizaki M, Ichida Y, Lex D, Kuroda E, Ishii KJ, Magi S, Okada M, Takao H, Gandou M,
et al
.
(13)
Selective Editing of Herpes Simplex Virus 1 Enables Interferon Induction and Viral Replication That Destroy Malignant Cells
Journal of Virology
January 15, 2019
Xing Liu, Bin He
perform
enzyme-linked immunosorbent assays
(
ELISA
), supernatants of
cell culture
were collected to analyze IFN-␣ (
42120-1
) and Cxcl9 (
DY492
) according to the manufacturer’s instructions (
R&D Systems
).
Transcriptome analysis
.
(14)
Topical essential fatty acid oil on wounds: Local and systemic effects
PloS one
January 1, 2019
Lania BG, Morari J, Almeida AR, Silva MND, Vieira-Damiani G, Lins KA, César CL, Velloso LA, Maia NB, Cintra ML,
et al
.
(15)
Flt3 ligand treatment reduces enterovirus A71 lethality in mice with enhanced B cell responses
Scientific reports
August 15, 2018
Lin YW, Wang LC, Lee CK, Chen SH.
(16)
The Essential Role of Double-Stranded RNA-Dependent Antiviral Signaling in the Degradation of Nonself Single-Stranded RNA in Nonimmune Cells
Journal of immunology (Baltimore, Md. : 1950)
August 1, 2018
Kimura S, Matsumiya T, Shiba Y, Nakanishi M, Hayakari R, Kawaguchi S, Yoshida H, Imaizumi T.
(17)
Initial Influenza Virus Replication Can Be Limited in Allergic Asthma Through Rapid Induction of Type III Interferons in Respiratory Epithelium
Frontiers in Immunology
May 17, 2018
Sujin An, Yung Jin Jeon, Ara Jo, Hyun Jung Lim, Young Eun Han, Sung Woo Cho, Hye Young Kim, Hyun Jik Kim
for variation between samples. Quantification of Secreted Cytokines The levels of secreted IFN-α (
42120-1
), IFN-β (
42400-1
), IFN-λ2/3 (
DY1789B
), and IFN-γ (
DY485
) were quantified using a Duoset ELISA kit (
R&D Systems
(18)
A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion
Immunity
April 17, 2018
Xia P, Wang S, Ye B, Du Y, Li C, Xiong Z, Qu Y, Fan Z.
(19)
The effect of royal jelly on the growth of breast cancer in mice
Oncology Letters
September 27, 2017
Shuang Zhang, Qiqi Shao, Haiyang Geng, Songkun Su
M4000B), Mouse IL‑10 Quantikine ELISA kit (cat no. M1000B),
Mouse
IFN‑α Quantikine ELISA kit (cat no.
42120‑1
) and
Mouse
IFN‑γ Quantikine ELISA kit (cat no.
MIF00
), according to the manufacturer's protocol, respectively.
(20)
TRAIL signaling is proinflammatory and proviral in a murine model of rhinovirus 1B infection
American journal of physiology. Lung cellular and molecular physiology
January 1, 2017
Girkin JL, Hatchwell LM, Collison AM, Starkey MR, Hansbro PM, Yagita H, Foster PS, Mattes J.
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Reviews
Be the First to Write a Review!
Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
IFN-alpha ELISA from R&D Systems, a Bio-Techne Brand
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Bench Tips
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